Recent studies have suggested that not only αβ⁺ T cells, but also the less common γδ⁺ T cells may play a role as effectors and immunoregolatory cells in the development and perpetuation of allergic inflammation. The objective of this study was to focus on the role of γδ⁺ T cells in atopic dermatitis (AD), a chronic relapsing inflammatory disease of the skin, often associated with allergic bronchial asthma. The present study employed flow cytometric analysis to compare numbers and phenotypic characteristics of γδ⁺ T cells in the peripheral blood of children with atopic dermatitis and age-matched healthy controls. The percentage of circulating Vγ 9Vδ2⁺ T lymphocytes was significantly increased in AD patients with respect to the age-matched controls, with a positive correlation with clinical score severity. The prevalent phenotype in both AD patients and controls was CD45RO⁺, with no differences observed in the percentage of Vδ2⁺ CD45RO⁺ between these groups. Conversely, memory CD45RO⁺ CD62L⁺ Vδ2⁺ lymphocytes were significantly lower in AD patients. Furthermore, naive circulating Vδ2⁺ T lymphocytes were significantly lower in AD children than in aged-matched controls. No correlation was observed between circulating Vγ 9Vδ2⁺ expansion and IgE serum levels. It was concluded that an association exists between the levels of circulating γδ⁺ T lymphocytes and atopic dermatitis, with a positive correlation with clinical score but no link with IgE serum levels. The pathophysiological role of γδ T lymphocytes in atopic dermatitis awaits further investigation.